Services

In general we provide the following services:

RNA-seq (full-length)

Single cells are FACS-sorted by the user into a 384-well plate prepared by ESCG. We do lysis, reverse transcription, and PCR followed by Smart-seq2 library preparation and sequencing (50 basepair, single-end).

RNA-seq, STRT-seq-2i (quantitative 5’tag)

Cells are either delivered to us as a single cell suspension that we dispense, or dispensed by FACS directly into lysis buffer on a WaferGen 9600 chip (WaferGen, Takara Bio Group). We do imaging (after dispensing), cell lysis, reverse transcription, and PCR followed by STRT library preparation and sequencing (50 basepair, single-read).

RNA-seq (quantitative 3’tag)

Cells are delivered to us as a single cell suspension and loaded on a Chromium chip (10XGenomics). We do cell lysis, reverse transcription, and PCR followed by Illumina library preparation and sequencing (200 cycles, paired-end).

RNA-seq (quantitative 5’tag) (discontinued)

Cells are delivered to us as a single cell suspension and loaded on a C1 chip (Fluidigm). We do imaging, cell lysis, reverse transcription, and PCR followed by STRT library preparation and sequencing (50 basepair, single-end).